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Sequencing procedures

The INIA Center for Research in Animal Health (CISA) has a specialized service in infectious animal diseases that carries out genomic sequencing studies.

For the development of these studies, INIA-CISA has High Biological Safety Laboratories of Biological Containment Level 3 and 3+ (NCB3 and NCB3 +).

INIA-CISA is a facility with open access to institutes, universities, laboratories, private companies and national and international public administration that investigate animal health.

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Genome sequencing reveals the sequence of nucleotides in a gene, nucleotides are organic molecules that form the building block of nucleic acids, RNA or DNA.

The INIA-CISA sequencing service enables the enhancement of molecular studies aimed at the detection, diagnosis and prevention of pathogens as well as those derived from basic research carried out by all INIA research staff.

It performs activities of automatic DNA sequencing, fragment analysis, massive sequencing, obtaining, purification and quantification of genomes, cloning of PCR products and in vitro transcription.

Characteristics

40 laboratories BSL-3 (WHO) and 2 laboratories BSL-4 (OIE)
15 independent experimental boxes at BSL-3 (WHO) for big and small animals and 4 boxes BSL-4 (OIE) adapted for in vivo experiments with zoonosis.
The independent nature of each box allows different simultaneous experiments to be carried out with conditions adapted to each type of experiment.
Boxes are prepared to house small and large animals, both farm and wild, adapting the space to the needs of each species according to animal welfare regulations.
Biosafety levels 3 (BSL3 (WHO)) and 3+ BSL (OIE)) allow the reproduction of animal diseases in vivo of importance in animal health and of a zoonotic nature.
INIA-CISA is the only research centre in Spain that can investigate foot-and-mouth disease in laboratories and animals.

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Frequent questions

How can genomic studies be requested to be carried out in the laboratories and high biological security animal facilities?

For open access to the network of high biosafety laboratories, the Public Access Protocol that describes the mechanism and criteria for access to the infrastructure must be followed (http://www.rlasb.es/protocolo-de-acceso/ ).

How can the request be formalized?

To formalize the request, you must have the support of a researcher working at INIA-CISA and request through secsgit@inia.es, with a copy to direccion.cisa@inia.es and delrio.lara@inia.es services from the Animal Research Centre to the General Subdirectorate for Research and Technology of the National Institute for Agricultural Research.

The email must indicate the name of the main researcher who will support the services in the INIA-CISA, the project to be carried out and the fiscal data of the company that will make the settlement effective (CIF and fiscal address).

Works can also be formalized through contracts and collaborations, in which an agreement must be reached that will be formalized in a contract.

Is a permit necessary for access to laboratories and animal facilities?

Access to the centre, laboratories and animal facilities is restricted, authorizations are granted to the different areas depending on the needs for the development of the works.

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Ask for more information

direccion.cisa@inia.es ; delrio.lara@inia.es
916202300